Figure 4. Immunofluorescence and apoptosis analysis of Hspa1l^+∕+ and Hspa1l^−∕− testes.

(A–D) The PLZF, γ-H2AX, PNA and Sox9 signals shows the locations of spermatogonia, spermatocytes, spermatids and Sertoli cells in testis sections from both Hspa1l^+∕+ and Hspa1l^−∕− mice, respectively. (E–F) Testicular sections of TUNEL assay in Hspa1l^+∕+ and Hspa1l^−∕− mice; (G) Average apoptotic cells per seminiferous tubule in Hspa1l^+∕+ and Hspa1l^−∕− testicular sections; (H) Average apoptotic cells per seminiferous tubules in Hspa1l^+∕+ and Hspa1l^−∕− testicular sections, n = 5.