Table 3. AutoSholl outputs similar results as human observers, but requires less time.
Values represent mean ± SEM. Dendrite lengths are in microns. Image Count: n2D = 5, n3D = 7.
| Observer A, Day 1 | Observer A, Day 2 | Observer B | Algorithm | |
|---|---|---|---|---|
| 2D Data | ||||
| Root Dendrite Count | 3.4 ± 0.2191 | 3.2 ± 0.1788 | 3.6 ± 0.2191 | 3.4 ± 0.2191 |
| Root Dendrite Length (μm) | 34.1807 ± 12.9296 | 35.2384 ± 12.6224 | 33.1338 ± 12.3609 | 22.9979 ± 11.2710 |
| Intermediate Dendrite Count | 2 ± 1.3856 | 2.4 ± 1.5126 | 2.4 ± 1.7111 | 2 ± 1.5748 |
| Intermediate Dendrite Length (μm) | 4.0109 ± 2.3496 | 4.2164 ± 1.9798 | 9.0545 ± 2.5783 | 2.5870 ± 1.4982 |
| Terminal Dendrite Count | 7 ± 1.5811 | 7.25 ± 1.8268 | 7.5 ± 2.2913 | 8 ± 1.8708 |
| Terminal Dendrite Length (μm) | 30.7466 ± 6.1412 | 27.7633 ± 6.1114 | 35.4112 ± 4.3202 | 30.01749 ± 3.8029 |
| Analysis Time (min) | 22.4833 ± 2.4009 | 22.6433 ± 2.6852 | 23.8033 ± 2.1885 | 0.6233 ± 0.0498* |
| 3D Data | ||||
| Root Dendrite Count | 8.4286 ± 1.0853 | 8.8571 ± 1.2194 | 8.4286 ± 1.1039 | 7.1429 ± 0.7403 |
| Root Dendrite Length (μm) | 6.4701 ± 0.8445 | 5.5941 ± 1.1697 | 5.2609 ± 1.0022 | 5.1681 ± 0.8709 |
| Intermediate Dendrite Count | 22.2857 ± 3.6181 | 21.2857 ± 4.4845 | 19.7143 ± 3.5669 | 20.1429 ± 3.3651 |
| Intermediate Dendrite Length (μm) | 4.0433 ± 0.5377 | 5.0214 ± 0.7517 | 4.5185 ± 0.5635 | 4.618 ± 0.6116 |
| Terminal Dendrite Count | 26.2857 ± 3.3728 | 24.7143 ± 2.9604 | 25.1429 ± 2.7362 | 24.7143 ± 3.3728 |
| Terminal Dendrite Length (μm) | 2.6478 ± 0.3114 | 2.7384 ± 0.3537 | 2.7363 ± 0.4445 | 2.3346 ± 0.4629 |
| Analysis Time (min) | 22.4264 ± 1.4904 | 24.7623 ± 1.7124 | 23.8888 ± 1.3271 | 12.2157 ± 0.6633* |
*
indicates significant differences from observers (p < 0.01).