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. 2020 Mar 26;15(3):e0230741. doi: 10.1371/journal.pone.0230741

Fig 1. CLUSTAL-W alignment of ITS2 regions from closely related Bactericera species showing variable regions and the gene target for the Bcoc_JSK2 primer and probe set.

Fig 1

Bases shades with black show differences to B. cockerelli sequence. Colour highlights locations of forward primer (blue highlight); reverse primer (green highlight) and probe (yellow highlight). The probe and reverse primer are reverse compliments of the highlighted regions here.