Table 1. Information on non-target psyllid species and plant specimens tested using the B. cockerelli real-time PCR assay Bcoc_JSK2 showing number of technical replicates and false positives.
| Family | Genus | Species | Voucher ID | Collection Location | Collection method | CO1 Ac# | ITS2 Ac# | Tech Reps | Voucher Location |
|---|---|---|---|---|---|---|---|---|---|
| Aphalaridae | Aphalara | avicularis | 160718.A.avi.23 | Wellesbourne, UK | suction trap | MT021761 | / | 2 | 1 |
| polygoni | 160718.A.pol.22 | Wellesbourne, UK | suction trap | / | MT038907 | 2 | 1 | ||
| Blastopsylla | occidentalis | 180312.Bl.occ.24 | Salamanca, Spain | suction trap | MN272146 | MN316692 | 2 | 3 | |
| Craspedolepta | gutierreziae | 160825.5 US | Nevada, USA | field collection | MT021786 | MT038962 | 2 | 1 | |
| minutissima | 160825.1 US | Nevada, USA | field collection | MT021787 | MT038963 | 2 | 1 | ||
| 160825.10 US | Oregon, USA | field collection | MT021789 | / | 2 | 1 | |||
| 160825.4 US | Nevada, USA | field collection | MT021788 | / | 2 | 1 | |||
| nervosa | 160728.Cra.ner.2 | Gogarbank, UK | suction trap | MT021790 | MT038964 | 2 | 1 | ||
| pinicola | 160825.2 US | Nevada, USA | field collection | / | MT038965 | 2 | 1 | ||
| subpunctata | 160421.C.sub.5 | Gogarbank, UK | suction trap | MT021791 | MT038966 | 2 | 1 | ||
| Rhinocola | aceris | 151014.R.ace.14 | Wellesbourne, UK | suction trap | MT021810 | MT038979 | 2 | 2 | |
| Liviidae | Diaphorina | citri | 160309.D.cit.6 | Lab Colony, Vietnam | Lab Reared | MT021794 | MT038969 | 2 | 1 |
| Euphyllura | olivina | 180125.Eup.oli.3 | imports from Italy | imported Olea europeae | MT021797 | MT038970 | 2 | 3 | |
| Livia | crefeldensis | 180312.L.cre.5 | Salamanca, Spain | suction trap | MN316678 | MN272127 | 2 | 3 | |
| junci | 160404.L.jun.1 | Broom' s Barn, UK | suction trap | MT021801 | / | 2 | 2 | ||
| opaqua | 160825.6 US | Nevada, USA | field collection | MT021802 | MT038973 | 2 | 1 | ||
| Psyllidae | Arytaina | genistae | 151203.A.gen.2J | Ayr, UK | suction trap | / | MT038909 | 2 | 1 |
| Arytainilla | gredi | 180312.A.gre.1 | Salamanca, Spain | suction trap | MN272123 | MN316677 | 2 | 3 | |
| spartiophila | 180716.A.spa.29 | Edinburgh, UK | suction trap | MT021762 | MT038908 | 2 | 3 | ||
| Baeopelma | foersteri | 151203.B.foe.1J | Ayr, UK | suction trap | / | MT038944 | 2 | 1 | |
| foersteri | 160928.B.foe.2 | SASA, UK | suction trap | MT021776 | / | 2 | 1 | ||
| Cacopsylla | affinis | 151203.C.aff.1 | Wye, UK | suction trap | MT021777 | MT038945 | 2 | 2 | |
| ambigua | 160404.C.amb.4 | Wye, UK | suction trap | / | MT038946 | 2 | 2 | ||
| ambigua | 161024.C.amb.3 | Preston, UK | suction trap | / | MT038947 | 2 | 1 | ||
| americana | 160825.3 US | Nevada, USA | field collection | MT021778 | MT038948 | 2 | 1 | ||
| brunneipennis | 160309.C.bru.8 | Wye, UK | suction trap | / | MT038949 | 2 | 2 | ||
| crataegi | 160404.C.cra.3 | Broom' s Barn, UK | suction trap | MT021779 | MT038950 | 2 | 2 | ||
| mali | 180910.C.mal.30 | Elcho, UK | field collection | / | MT038951 | 2 | 3 | ||
| melanoneura | 160718.C.mel.6 | Kirton, UK | suction trap | / | MT038952 | 2 | 3 | ||
| moscovita | 190109.C.mos.1 | Germany | suction trap | / | / | 2 | 3 | ||
| peregrina | 161024.C.per.11 | Silwood Park, UK | suction trap | MT021780 | MT038953 | 2 | 1 | ||
| pruni | 160203.C.pru.18 | Wellesbourne, UK | suction trap | / | MT038954 | 2 | 2 | ||
| pulchra | 160718.C.pul.15 | Elgin, UK | suction trap | / | MT038955 | 2 | 1 | ||
| pyricola | 160203.C.pco.2 | Wye, UK | suction trap | MT021781 | MT038956 | 2 | 2 | ||
| saliceti | 161024.C.sal.7 | York, UK | suction trap | / | MT038958 | 2 | 1 | ||
| sorbi | 161024.C.sor.8 | Preston, UK | suction trap | MT021782 | MT038959 | 2 | 1 | ||
| rhamnicola | 151014.C.rha.8 | Wellesbourne, UK | suction trap | / | MT038957 | 2 | 2 | ||
| ulmi | 171011.C.ulm.13 | Germany | suction trap | MT021783 | MT038960 | 2 | 3 | ||
| Ceanothia | ceanothi | 160825.9 US | Oregon, USA | field collection | MT021784 | / | 2 | 1 | |
| Chamaepsylla | hartigii | 160728.Ch.har.1 | Gogarbank, UK | suction trap | MT021785 | MT038961 | 2 | 1 | |
| Euglyptoneura | fuscipennis | 160825.7 US | Oregon, USA | field collection | MT021795 | / | 2 | 1 | |
| robusta | 160825.8 US | Oregon, USA | field collection | MT021796 | / | 2 | 1 | ||
| Heteropsylla | texana | 160825.11 US | Texas, USA | field collection | MT021798 | / | 2 | 1 | |
| Psylla | alni | 161019.P.aln.1 | Sweden | suction trap | MT021804 | / | 2 | 1 | |
| buxi | 180622.P.bux.22 | Scotland, UK | suction trap | MT021806 | MT038976 | 2 | 3 | ||
| betulae | 161123.P.bet.20 | Jokioinen, Finland | suction trap | MT021805 | MT038975 | 2 | 3 | ||
| Psyllopsis | discrepans | 151002.P.dis.8 | Sweden | suction trap | MT021807 | / | 2 | 1 | |
| fraxini | 180716.P.fri.33 | Edinburgh, UK | suction trap | MT021808 | MT038977 | 2 | 3 | ||
| fraxinicola | 160203.P.fra.6 | Wellesbourne, UK | suction trap | MT021809 | MT038978 | 2 | 2 | ||
| Spanioneura | fonscolombii | 180802.S.fon.29 | Edinburgh, UK | field collection | / | MT038980 | 2 | 3 | |
| Spondyliaspidae | Ctenarytaina | spatulata | 160404.Ct.spa.6 | Wye, UK | suction trap | MT021792 | MT038967 | 2 | 2 |
| spatulata | 161024.Ct.spa.5 | Wye, UK | suction trap | MT021793 | MT038968 | 2 | 1 | ||
| Triozidae | Bactericera | albiventris | 171214.B.alb.11 | Jokioinen, Finland | suction trap | / | MT038910 | 5 | 3 |
| curvatinervis | 161123.B.cur.42 | Jokioinen, Finland | suction trap | / | MT038911 | 5 | 3 | ||
| dorsalis | 160803.B.dor.2 | Florida, USA | lab colony | MT021763 | MT038912 | 5 | 3 | ||
| maculipennis | 190604.B.mac.1 | Lab Colony, USA | Lab Reared | / | MT038913 | 2 | 3 | ||
| 190604.B.mac.2 | Lab Colony, USA | Lab Reared | / | MT038914 | 2 | 3 | |||
| 190604.B.mac.3 | Lab Colony, USA | Lab Reared | / | MT038915 | 2 | 3 | |||
| 190604.B.mac.4 | Lab Colony, USA | Lab Reared | / | MT038916 | 2 | 3 | |||
| 190604.B.mac.5 | Lab Colony, USA | Lab Reared | / | MT038917 | 2 | 3 | |||
| 190604.B.mac.6 | Lab Colony, USA | Lab Reared | / | MT038918 | 2 | 3 | |||
| 190604.B.mac.7 | Lab Colony, USA | Lab Reared | / | MT038919 | 2 | 3 | |||
| nigricornis | 170324.B.nig.18 | Spain | field collection | MT021764 | MT038920 | 5 | 3 | ||
| 170324.B.nig.22 | Spain | field collection | MT021765 | MT038921 | 5 | 3 | |||
| salicivora | 190116.B.sal.1 | Elgin, UK | suction trap | / | / | 6 | 3 | ||
| striola | 161123.B.str.9 | Jokioinen, Finland | suction trap | / | MT038922 | ||||
| tremblayi | 170731.B.tre.5 | Belgrade, Serbia | field collection | / | MT038923 | 5 | 3 | ||
| 190604.B.tre.17 | Spain | Lab Colony | / | MT038924 | 2 | 3 | |||
| 190604.B.tre.18 | Spain | Lab Colony | / | MT038925 | 2 | 3 | |||
| 190604.B.tre.19 | Spain | Lab Colony | / | MT038926 | 2 | 3 | |||
| 190604.B.tre.20 | Spain | Lab Colony | / | MT038927 | 2 | 3 | |||
| 190604.B.tre.21 | Spain | Lab Colony | / | MT038928 | 2 | 3 | |||
| trigonica | 170629.B.tri.16 | Tunisia | field collection | MT021766 | MT038929 | 3 | 3 | ||
| 170629.B.tri.17 | Tunisia | field collection | / | MT038930 | 3 | 3 | |||
| 170629.B.tri.18 | Tunisia | field collection | MT021767 | MT038931 | 3 | 3 | |||
| 181010.B.tri.17 | Spain | Lab Colony | MT021768 | MT038932 | 2 | 3 | |||
| 181010.B.tri.18 | Spain | Lab Colony | MT021769 | MT038933 | 2 | 3 | |||
| 181010.B.tri.19 | Spain | Lab Colony | / | MT038934 | 2 | 3 | |||
| 181010.B.tri.20 | Spain | Lab Colony | MT021770 | MT038935 | 2 | 3 | |||
| 181010.B.tri.21 | Spain | Lab Colony | / | MT038936 | 2 | 3 | |||
| 190604.B.tri.23 | Spain | Lab Colony | MT021771 | MT038937 | 2 | 3 | |||
| 190604.B.tri.24 | Spain | Lab Colony | / | MT038938 | 2 | 3 | |||
| 190604.B.tri.25 | Spain | Lab Colony | MT021772 | MT038939 | 2 | 3 | |||
| 190604.B.tri.26 | Spain | Lab Colony | MT021773 | MT038940 | 2 | 3 | |||
| 190604.B.tri.27 | Spain | Lab Colony | MT021774 | MT038941 | 2 | 3 | |||
| 190604.B.tri.28 | Spain | Lab Colony | / | MT038942 | 2 | 3 | |||
| 190604.B.tri.29 | Spain | Lab Colony | MT021775 | MT038943 | 2 | 3 | |||
| Heterotrioza | chenopodii | 160203.H.che.11 | Kirton, UK | suction trap | / | MT038971 | 2 | 2 | |
| 160825.12 US | Washington, USA | field collection | MT021799 | / | 2 | 1 | |||
| Lauritrioza | alacris | 160816.L.ala.2 | Spain | suction trap | MT021800 | MT038972 | 2 | 1 | |
| Powellia | vitreoradiata | 161024.P.vit.10 | Kirton, UK | suction trap | MT021803 | MT038974 | 2 | 1 | |
| Trioza | albifrons | 160825.18.US | Nevada, USA | field collection | MT021811 | MT038981 | 2 | 1 | |
| anthrisci | 150708.T.ant.11 | Jokioinen, Finland | field collection | MT021812 | / | 2 | 3 | ||
| apicalis | 161019.T.api.5 | Sweden | field collection | MT021813 | / | 2 | 3 | ||
| buxtoni | 170324.T.bux.11 | Israel | field collection | MT021814 | MT038982 | 2 | 3 | ||
| centranthi | 161024.T.cen.9 | Wye, UK | suction trap | MT021815 | / | 2 | 1 | ||
| cerastii | 171214.T.cer.32 | Vikki, Finland | suction trap | MT021816 | MT038983 | 2 | 3 | ||
| dispar | 160718.T.dis.26 | Hellfreda, Sweden | suction trap | MT021817 | / | 2 | 1 | ||
| erytreae | 160808.ICA.19 | Spain | Lab Colony | / | MT038984 | 2 | 1 | ||
| flavipennis | 160421.T.fla.3 | Sweden | suction trap | MT021818 | MT038985 | 2 | 1 | ||
| galii | 160203.T.gal.23 | Wellesbourne, UK | suction trap | / | MT038986 | 2 | 2 | ||
| remota | 160718.T.rem.8 | Sweden | suction trap | / | MT038987 | 2 | 1 | ||
| 180424.T.rem.1 | Dundee, UK | Suction trap | MT021819 | MT038988 | 3 | 3 | |||
| 180424.T.rem.6 | Dundee, UK | Suction trap | MT021820 | MT038989 | 3 | 3 | |||
| 180424.T.rem.16 | Dundee, UK | Suction trap | MT021821 | MT038990 | 3 | 3 | |||
| 180424.T.rem.18 | Dundee, UK | Suction trap | MT021822 | MT038991 | 3 | 3 | |||
| 180424.T.rem.19 | Dundee, UK | Suction trap | / | MT038992 | 3 | 3 | |||
| 190116.T.rem.7 | UK | Suction trap | MT021823 | MT038993 | 3 | 3 | |||
| rhamni | 151002.T.rha.13 | Sweden | suction trap | MT021824 | MT038994 | 2 | 1 | ||
| tatrensis | 160718.T.tat.27 | Sweden | suction trap | / | MT038995 | 2 | 1 | ||
| urticae | 160816.T.urt.17 | Spain | field collection | / | MT038996 | 2 | 1 |
All non-target species gave 0% false positives. GenBank Accession numbers are included for ITS2 and CO1 regions if sequencing was successful. Voucher Location: 1 = 1; 2 = 2 Research Insect Survey; 3 = SASA Hemipteran DNA Database. All DNA samples are stored in the SASA Hemipteran DNA database. “/” = no sequence obtained.