Table 4. Information on Bactericera cockerelli samples tested with Bcoc_JSK2 assay including genomic DNA from adults, immatures, single eggs and egg batches.
Location of samples collection is also included. All samples gave 100% positives. Accession numbers for CO1 and ITS2 (MT027551-MT027599) regions are included. “/” = no sequence obtained.
| Sample name | Life Stage | Origin | Ct ave | Tech reps | CO1 Ac# | ITS2 Ac# | DNA Source |
|---|---|---|---|---|---|---|---|
| 181119.B.coc.06 | 1 egg | Mexico | 29.80 | 2 | / | MT027568 | Genomic |
| 191003.B.coc.01 | 1 egg | Mexico | 33.41 | 3 | / | MT027592 | Genomic |
| 191003.B.coc.02 | 1 egg | Mexico | 24.95 | 3 | / | MT027593 | Genomic |
| 191003.B.coc.03 | 1 egg | Mexico | 33.79 | 3 | / | MT027594 | Genomic |
| 191003.B.coc.04 | 1 egg | Mexico | 22.43 | 6 | / | MT027595 | Genomic |
| 181119.B.coc.07 | 5 eggs | Mexico | 24.42 | 2 | / | MT027569 | Genomic |
| 181119.B.coc.21 | 5 eggs | Mexico | 28.32 | 2 | / | MT027582 | Genomic |
| 181119.B.coc.08 | 10 eggs | Mexico | 29.61 | 2 | / | MT027570 | Genomic |
| 181119.B.coc.22 | 10 eggs | Mexico | 26.43 | 2 | / | MT027583 | Genomic |
| 181119.B.coc.03 | immature | Mexico | 22.56 | 2 | / | MT027565 | Genomic |
| 181119.B.coc.04 | immature | Mexico | 22.33 | 2 | / | MT027566 | Genomic |
| 181119.B.coc.05 | immature | Mexico | 21.46 | 2 | / | MT027567 | Genomic |
| 181119.B.coc.11 | immature | Mexico | 23.16 | 2 | / | MT027573 | Genomic |
| 181119.B.coc.12 | immature | Mexico | 24.15 | 2 | / | MT027574 | Genomic |
| 181119.B.coc.13 | immature | Mexico | 23.94 | 2 | / | MT027575 | Genomic |
| 181119.B.coc.14 | immature | Mexico | 25.75 | 2 | / | MT027576 | Genomic |
| 181119.B.coc.16 | immature | Mexico | 23.49 | 2 | / | MT027578 | Genomic |
| 181119.B.coc.18 | immature | Mexico | 22.45 | 2 | / | MT027580 | Genomic |
| 181119.B.coc.19 | immature | Mexico | 23.50 | 2 | / | MT027581 | Genomic |
| 190604.B.coc.13 | immature | Mexico | 24.96 | 2 | / | MT027588 | Genomic |
| 190604.B.coc.14 | immature | Mexico | 25.09 | 2 | / | MT027589 | Genomic |
| 190604.B.coc.15 | immature | Mexico | 28.37 | 2 | / | MT027590 | Genomic |
| 150727.B.coc.02 | Adult | South Western, USA | 22.18 | 2 | MT040955 | MG719775 | Genomic |
| 150827.B.coc.02 | Adult | South Western, USA | 22.18 | 2 | MT040956 | MT027597 | Genomic |
| 150827.B.coc.03 | Adult | Central USA | 24.49 | 6 | MT040957 | MT027598 | Genomic |
| 150827.B.coc.04 | Adult | North Western, USA | 24.77 | 2 | MT040958 | MT027599 | Genomic |
| 150827.B.coc.06 | Adult | North Western, USA | 23.68 | 2 | MT040960 | MT027552 | Genomic |
| 150827.B.coc.12 | Adult | Western, USA | 20.39 | 2 | MT040961 | MT027596 | Genomic |
| 150827.B.coc.17 | Adult | South Western, USA | 19.65 | 2 | MT040962 | MT027553 | Genomic |
| 160725.B.coc.05 | Adult | Central, USA | 21.45 | 2 | MT040963 | / | Genomic |
| 160726.B.coc.01 | Adult | New Zealand | 21.56 | 2 | / | MT027557 | Genomic |
| 160726.B.coc.02 | Adult | New Zealand | 21.02 | 2 | / | MT027558 | Genomic |
| 160726.B.coc.03 | Adult | New Zealand | 20.48 | 2 | / | MT027559 | Genomic |
| 160726.B.coc.04 | Adult | New Zealand | 21.98 | 2 | / | MT027560 | Genomic |
| 160726.B.coc.05 | Adult | New Zealand | 19.43 | 2 | / | MT027561 | Genomic |
| 160726.B.coc.06 | Adult | New Zealand | 20.96 | 2 | / | MT027562 | Genomic |
| 180731.B.coc.04 | Adult | North Western, USA | 24.42 | 6 | MT040964 | / | Genomic |
| 180731.B.coc.05 | Adult | Western, USA | 22.91 | 6 | MT040965 | / | Genomic |
| 180731.B.coc.06 | Adult | Western, USA | 27.14 | 6 | MT040966 | / | Genomic |
| 181119.B.coc.01 | Adult | Mexico | 21.47 | 2 | / | MT027563 | Genomic |
| 181119.B.coc.02 | Adult | Mexico | 19.98 | 2 | / | MT027564 | Genomic |
| 181119.B.coc.09 | Adult | Mexico | 21.83 | 2 | / | MT027571 | Genomic |
| 181119.B.coc.10 | Adult | Mexico | 19.48 | 2 | / | MT027572 | Genomic |
| 181119.B.coc.15 | Adult | Mexico | 21.27 | 2 | / | MT027577 | Genomic |
| 181119.B.coc.17 | Adult | Mexico | 23.74 | 2 | / | MT027579 | Genomic |
| 190604.B.coc.09 | Adult | USDA, Lab Colony | 21.51 | 2 | / | MT027584 | Genomic |
| 190604.B.coc.10 | Adult | Mexico | 20.33 | 2 | / | MT027585 | Genomic |
| 190604.B.coc.11 | Adult | Mexico | 22.67 | 2 | / | MT027586 | Genomic |
| 190604.B.coc.12 | Adult | Mexico | 24.37 | 2 | / | MT027587 | Genomic |
| 190604.B.coc.16 | Adult | Mexico | 27.15 | 2 | / | MT027591 | Genomic |
| 150827.B.coc.05.col.04 | transformed E. coli | Lab | 11.23 | 6 | MT040959 | MT027551 | Cloned, 10ng |
| 160725.B.coc.01.col.06 | transformed E. coli | Lab | 11.55 | 6 | / | MT027554 | Cloned, 10ng |
| 160725.B.coc.06.col.04 | transformed E. coli | Lab | 11.78 | 6 | / | MT027555 | Cloned, 10ng |
| 160725.B.coc.07.col.08 | transformed E. coli | Lab | 11.67 | 6 | / | MT027556 | Cloned, 10ng |