Skip to main content
. 2020 Mar 26;15(3):e0229672. doi: 10.1371/journal.pone.0229672

Fig 1. Characterization of MBP-tagged HPV18 E6 protein via SDS-PAGE, size-exclusion chromatography, and mass spectrometry.

Fig 1

A–SDS-PAGE gel of MBP-trap purification of pMal MBP-18E6. Lane 1 designated the molecular weight marker, lane 2 is protein loaded on the column, lane 3 is the flow-through from the column, lanes 4 and 5 are independent collections of the peak of the primary elution, lane 6 is empty, lanes 7–9 is raw load protein (7), column flow-through (8), and peak of the primary elution (9) subjected to DTT reduction. B–SDS-PAGE gel of MBP-HPV18 E6 protein prior to and following DTT reduction with a Hi trap Q anion exchange column. Lane 1 designated the molecular weight marker, lane 2 is protein loaded on the column, lane 3 is the flow-through from the column, lanes 4 and 5 are independent collections of the peak of the primary elution, lanes 6–8 is raw load protein (6), column flow-through (7), and peak of the primary elution (8) subjected to DTT reduction. C–Size-exclusion chromatography of the peak of the primary elution subjected to DTT reduction in Fig 1A. D–Mass spectrometry of MBP-HPV18 E6 protein from re-folded and reduced, peak eluate solution from the QHP column.