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. 2020 Mar 26;15(3):e0229672. doi: 10.1371/journal.pone.0229672

Fig 5. Sample-dependent and reference material dilutional linearity in the multiplex anti-HPV MSD assay.

Fig 5

Serum from HPV+ cervical cancer subjects (n = 3), and normal, healthy adult donors (n = 4) was evaluated to identify the linear range for sample testing over 2 assays. Concentration for each sample was normalized to the observed concentration at 800-fold and plotted as a percent change from this value across 8 dilutions. A–dilutional linearity for seven samples in relation to the reference standard for anti-HPV16 E6 concentration. B–dilutional linearity for seven samples in relation to the reference standard for anti-HPV16 E7 concentration. C–dilutional linearity for seven samples in relation to the reference standard for anti-HPV18 E6 concentration. D–dilutional linearity for seven samples in relation to the reference standard for anti-HPV18 E7 concentration. E–Dilutional linearity of the reference serum over three assays runs plotted as ECL signal (left axis; solid lines) or percent recovery (right axis; dashed lines) ± %CV as a function of dilution factor of serum.