Table 1. Concentration assignment of the pooled human donor reference serum from observed ECL signal.

	HPV16 E6		HPV16 E7		HPV18 E6		HPV18 E7
Dilution factor	ECL Signal	Assigned Concentration (AU/mL)	ECL Signal	Assigned Concentration (AU/mL)	ECL Signal	Assigned Concentration (AU/mL)	ECL Signal	Assigned Concentration (AU/mL)
100	402403	30	88255	5	623159	50	315200	20
400	107323	7.5	23485	1.25	185969	12.5	77691	5
1600	28967	1.875	4541	0.313	51975	3.125	20165	1.250
6400	7218	0.469	956	0.078	13054	0.781	4689	0.313
25600	1832	0.117	330	0.020	3378	0.195	1269	0.078
102400	588	0.029	152	0.005	1131	0.049	369	0.020
409600	179	0.007	92	0.001	510	0.012	148	0.005
Blank	62		91		316		54