Table 4. Effect of T-2 toxin on oxidative stress enzymes in various organs.
Organ | Enzymes | (Days) | Groups | F value | ||
---|---|---|---|---|---|---|
10ppm (Group I) | 20ppm (Group II) | Control (III) | ||||
Liver | CAT (103/U) | 15 | 1756.50±3.15aB | 1684.2±9.45bB | 1629.2±1.95cB | FG = 226.4** |
FD = 305.7** | ||||||
FDG = 88.85** | ||||||
30 | 1843.90±5.70bA | 1939.4±17.8aA | 1659.3±1.9cA | |||
SOD (U/g) | 15 | 77.10±1.25bB | 86.6±2.30aB | 73.2±0.55bB | FG = 72.32** | |
30 | 106.60±4.05aA | 118.8±1.6aA | 82.9±0.80bA | FD = 187.0** | ||
FDG = 17.0** | ||||||
LPO (nM) | 15 | 56.00±2.65aB | 63.4±4.15a | 33.9±1.4b | FG = 90.9** | |
30 | 78.80±4.40aA | 80.9±4.15a | 29.8±0.5b | FD = 20.2** | ||
FDG = 9.45* | ||||||
Intestine | CAT (103/U) | 15 | 1578.30±4.95bB | 1804.6±5.8aB | 1436.3±2.4cB | FG = 566.0** |
30 | 2037.70±6.5aA | 1949.8±24.8bA | 1619.3±4cA | FD = 854.9** | ||
FDG = 121.8** | ||||||
SOD (U/g) | 15 | 85.90±0.35bB | 110.8±1.55aB | 76.5±0.45c | FG = 422.6** | |
30 | 106.30±2.05bA | 134.4±2.15aA | 78.6±1.65c | FD = 123.5** | ||
FDG = 30.9** | ||||||
LPO (nM) | 15 | 57.40±3.10bB | 96.3±3.6a | 48.1±2.55b | FG = 206.5** | |
30 | 98.40±2.0aA | 113.7±4.0a | 41.7±1.9b | FD = 51.0** | ||
FDG = 32.1** | ||||||
MLN | CAT (103/U) | 15 | 1602.80±9.4bB | 1649±4.65aB | 1366.1±3.4cB | FG = 1009.6** |
30 | 1665.20±8.7bA | 1751.8±6.05aA | 1482.4±3.2cA | FD = 331.1** | ||
FDG = 10.5* | ||||||
SOD (U/g) | 15 | 74.80±2.45aB | 78.6±0.85aA | 45.6±2.15b | FG = 266.5** | |
30 | 86.0±1.55aA | 70.5±1.05bB | 52.2±0.9c | FD = 11.3* | ||
FDG = 24.9** | ||||||
LPO (nM) | 15 | 56.4±1.45B | 58.6±1.05B | 50.9±3.7 | FG = 30.3** | |
30 | 70.4±2.45aA | 79.4±3.45aA | 50.3±1.35b | FD = 35.0** | ||
FDG = 8.7* | ||||||
Kidneys | CAT (103/U) | 15 | 1717.60±15.20b | 1789.4±7.2aA | 1571.6±2.4bA | FG = 78.8** |
30 | 1717.00±6.40a | 1651.5±3.8bB | 1590.6±1.35cB | FD = 150.7** | ||
FDG = 41.9** | ||||||
SOD (U/g) | 15 | 74.80±2.45b | 116.4±3.00a | 71.2±1.1c | FG = 292.6** | |
30 | 84.60±2.25b | 135.3±3.5a | 66.5±2.1c | FD = 15.6** | ||
FDG = 11.7** | ||||||
LPO (nM) | 15 | 60.50±3.9 | 61.4±2.5 | 49.6±4.35 | FG = 16.95** | |
30 | 73.50±2.55 | 61.9±4.35 | 48.1±6.2 | FD = 0.8 | ||
FDG = 3.88 | ||||||
Spleen | CAT (103/U) | 15 | 1431.20±1.65cB | 1766.3±4.45aB | 1573.8±2.5bB | FG = 1904.4** |
FD = 944.3** | ||||||
FDG = 197.6** | ||||||
30 | 1642.80±3.55bA | 1829.3±8.55aA | 1630.2±1.4bA | |||
SOD (U/g) | 15 | 67.30±0.95bB | 81.3±1.95a | 56.9±2.6b | FG = 78.7** | |
30 | 73.40±1.00bA | 89.3±0.95a | 65.3±3.05b | FD = 22.5** | ||
FDG = 0.22 | ||||||
LPO (nM) | 15 | 70.40±2.75b | 91.4±1.75aB | 74.9±2.75b | FG = 65.99** | |
30 | 74.40±4.80b | 122.2±3.8aA | 81.25±2.05b | FD = 27.5** | ||
FDG = 10.6* | ||||||
Brain | CAT (103/U) | 15 | 1626.60±12.90bB | 1717.7±5.6aB | 1564.9±2.75cA | FG = 1675.2** |
FD = 9.84* | ||||||
30 | 1717.50±5.80bA | 1909.7±1.4aA | 1329.2±1.6cB | FDG = 610** | ||
SOD (U/g) | 15 | 72.40±0.75bB | 94.2±1.00a | 65.1±2.2b | FG = 115.9** | |
30 | 82.20±0.70abA | 88.30±1.05a | 74.2±2.1b | FD = 14.2** | ||
FDG = 19.23* | ||||||
LPO (nM) | 15 | 79.90±3.25b | 99.40±1.65a | 77.9±3.8b | FG = 33.9** | |
30 | 82.40±5.40b | 108.30±2.25a | 79.3±2.45b | FD = 2.7 | ||
FDG = 0.81 |
The data were analysed using one way ANOVA, two way ANOVA and independent t test wherever applicable. Superscripts A, B and C shows differences in columns and a, b, c, d in rows. Mean bearing at least one common superscript didn’t show any difference. FD = effect within days (D), FG = effect within groups (G), FDG = interaction within days and groups *<0.05, **<0.01. CAT (103/U)—Catalase, SOD-Superoxide Dismutase, LPO-lipid peroxidation (nM of MDA/g of tissue).