Table 11. Effect of T-2 toxin on expression of pro-inflammatory cytokine genes in different tissues in 10 ppm and 20 ppm groups on 30th day.
| Tissues | Genes | Groups | Fold change (2-ΔΔct) ±SEM | P value |
|---|---|---|---|---|
| Liver | IL-1α | 10ppm | 13.85±2.40* | 0.04 |
| 20ppm | 36.37±4.47* | |||
| IL-6 | 10ppm | 3.62±0.18# | 0.05 | |
| 20ppm | 9.54±0.93# | |||
| TNF-α | 10ppm | 23.85±1.87$ | 0.05 | |
| 20ppm | 13.29±1.63$ | |||
| Intestine | IL-1α | 10ppm | 60.15±4.28* | 0.05 |
| 20ppm | 84.25±4.15* | |||
| IL-6 | 10ppm | 11.11±1.01# | 0.04 | |
| 20ppm | 26.08±4.03# | |||
| TNF-α | 10ppm | 42.12±5.78$ | 0.05 | |
| 20ppm | 56.13±1.18$ | |||
| MLN | IL-1α | 10ppm | 11.72±1.28 | 0.16 |
| 20ppm | 7.85±1.10 | |||
| IL-6 | 10ppm | 1.82±0.58 | 0.58 | |
| 20ppm | 4.22±0.13 | |||
| TNF-α | 10ppm | 13.72±2.19$ | 0.04 | |
| 20ppm | 6.36±0.46$ | |||
| kidneys | IL-1α | 10ppm | 2.14±0.17 | 0.12 |
| 20ppm | 4.28±0.48 | |||
| IL-6 | 10ppm | 2.68±0.89# | 0.04 | |
| 20ppm | 10.95±1.45# | |||
| TNF-α | 10ppm | 19.97±1.13$ | 0.05 | |
| 20ppm | 8.69±0.88$ | |||
| Spleen | IL-1α | 10ppm | 17.72±1.47* | 0.05 |
| 20ppm | 25.4±4.76* | |||
| IL-6 | 10ppm | 10.19±0.79## | 0.001 | |
| 20ppm | 52.23±7.59## | |||
| TNF-α | 10ppm | 49.92±4.30$ | 0.05 | |
| 20ppm | 12.59±1.22$ | |||
| Brain | IL-1α | 10ppm | 1.78±0.50* | 0.05 |
| 20ppm | 4.73±0.31* | |||
| IL-6 | 10ppm | 2.42±0.46 | 0.32 | |
| 20ppm | 3.61±0.28 | |||
| TNF-α | 10ppm | 3.83±1.04 | 0.17 | |
| 20ppm | 2.17±0.49 |
The relative fold change (2-ΔΔct) expressions of 10 ppm and 20 ppm groups were compared relative to control on 30th day using Mann–Whitney U-test as implemented in version 16.0 of the SPSS software. The level of significance was set at P < 0.05.