(A, C, E) Jurkat-based HIV-1 latency cell lines, J-Lat 10.6 (A) and EF7 (C), as well as a monocytic one, U1/HIV (E), were stably transduced with the indicated shRNA (shNT or shNOP2) in pAPM vector, and NOP2 knockdown was analyzed by RT-qPCR. (B, D) Jurkat-based J-Lat 10.6 (B) and EF7 (D) cells stably expressing shNT or shNOP2 were treated with DMSO, SAHA (1 uM), JQ1 (0.5 uM), or Prostratin (0.5 uM), to reactivate latent HIV-1. Percentage of GFP-expressing cells was determined by flow cytometry. (E) U1/HIV cells stably expressing shNT or shNOP2 were stimulated with DMSO, TNF-α (10 ng/ml), JQ1 (0.5 uM), or Prostratin (0.5 uM), to reactivate latent HIV-1. Total RNA was extracted, followed by RT-qPCR to measure the mRNA level of HIV-1 Gag, which was normalized to that of shNT. Results were based on n = 3 experiments and presented as mean ± S.D., * p < 0.05; ** p < 0.01; *** p < 0.001, ANOVA.