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. 2020 Mar 16;14(3):e0008093. doi: 10.1371/journal.pntd.0008093

Fig 9. Percentages of CD8+ T cells producing IFN-γ in response to selected multi-epitope peptide pools.

Fig 9

PBMC from cured CL subjects (N = 9), previously stimulated with selected peptide pools (1 μM/peptide) for 10 days, were re-stimulated overnight with the same peptide pools (1 μM/peptide) and with anti-CD49d/anti-CD28 antibodies (1 μg/ml). For intracellular IFN-γ detection, cells were treated with Golgistop for 6 h of culture, fixed and permeabilized using BD Cytoperm/cytofix kit. Data were analyzed by FlowJo software. PBMC stimulated with PMA (50 ng/ml)/Ionomicyn (10–6M) for 6 h or SLA (10 μg/ml) for 5 days, were used as positive control. Results represent the frequency of CD8+ IFN-γ+ T cell populations. Horizontal bars represent median values. Statistical significance was assigned to a value of p<0,05 (*p<0.05, **p<0.01).