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. 2020 Mar 16;16(3):e1008703. doi: 10.1371/journal.pgen.1008703

Fig 1. The absence of the V. cholerae flagellum filament elicits a flagellum-dependent biofilm regulatory response.

Fig 1

A) Illustration showing the main components of the polar flagellum in V. cholerae with the proteins forming these components shown in brackets. The flagellum sheath is not depicted in this figure. The structures targeted in this study are color coded. B) Representative images of the smooth colony morphology of the WT strain and the corrugated colony morphology of the ΔflaA strain. Scale bars = 1 mm. C) Bar graph of means and standard deviations of relative luminescent units (RLU) obtained from the transcription of vpsL-luxCDABE in colonies of the WT and ΔflaA strains. D) Bar graph of means and standard deviations of c-di-GMP concentration measured by LC-MS/MS in colonies of the WT and ΔflaA strains. E) Bar graph of means and standard deviations of RLU obtained from the expression of the c-di-GMP biosensor in colonies of the WT and ΔflaA strains. Means obtained from three biological replicates were compared with an unpaired t-test. Mean differences with a P value ≤ 0.05 were deemed significant. **** p ≤ 0.0001.