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. 2020 Jan 30;5(2):e131437. doi: 10.1172/jci.insight.131437

Figure 4. Recombinantly expressed V2-specific mAb epitope mapping by ELISA.

Figure 4

Recombinantly expressed mAbs were assayed by ELISA. (A) Three groups of V1V2-specific antibodies were identified: those that bound to V2 linear peptide, those that were conformational dependent, and those that were glycan dependent. The (B) linear peptide–binding, (C) conformational dependent, (D) and glycan-dependent mAbs were epitope mapped on AE.A244gp120Δ11 with point mutations in the V1V2 loop. Percentage of binding to the mutant AE.A244gp120Δ11 relative to the WT AE.A244gp120Δ11. Values are the mean of 2 independent experiments.