TABLE 4.
Primer pair and probe nucleotide sequences for associated qPCR assay with resulting amplicon size
| Singleplex or multiplex qPCR assay | Amplicon size (bp) | Primers and associated probe identification | Primer (5′ to 3′) |
|---|---|---|---|
| U16S | 159 | U16s_forward primer | TGGAGCATGTGGTTTAATTCGA |
| U16s_reverse primer | TGCGGGACTTAACCCAACA | ||
| U16s_probe | CACGAGCTGACGACARCCRTGCA | ||
| 4Pan1 | 116 | 4pan1_Forward primer | CAYCCTAGACTATTCTATACTTAC |
| 4pan1_Reverse primer | GTAAATCTATTTACTTGAAACATCTGC | ||
| 4pan1_Probe | CCGTACCAAGATCAAACAAATATACC | ||
| 3Pan | 83 | 3pan_Forward primer | TTTACACCCGTCTCCGTTAGT |
| 3pan_Reverse primer | CTCTTAAGGATGCAATTTGGGATT | ||
| 3pan_Probe | AAGAGGCAAAGCTGGAATTACACTCTCTC | ||
| A1d | 114 | A1d_forward primer | CACCCAGCAACAAAGTAGCAC |
| A1d_reverse primer | CTATCTCATCATCAAAATCTATAAGAGC | ||
| A1d_probe | CTCTTGCTGTTTTTTTAGCTGGATTATCC | ||
| A2c | 101 | A2c_forward primer | GGCTTTGCTAGCACAAATAAACC |
| A2c_reverse primer | GATAAACAGCAATTCTTTAAGACGAC | ||
| A2c_probe | CACTGTTAGTGACAATCCCTGCTATAG | ||
| B2 | 80 | B2_forward primer | CCTATCCAATACTCCGAGTTAGT |
| B2_reverse primer | AAATCAAAAGAAGAGTTAAAACAAGC | ||
| B2_probe | CTCTGGCCAGTTATTTTTATCAAAGCCAG | ||
| M3 | 112 | M3_forward primer | AGCACATGCTAGTTTAATGAGTT |
| M3_reverse primer | ACTAGTTGATGCAGAGTTACC | ||
| M3_probe | CTACACCCATTTGGGAAATGCCTTC | ||
| N1 | 140 | N1_forward primer | CTTGTTGTGGTAAAAATAGCTTAG |
| N1_reverse primer | GGAAGTTTTCATGAGTAAGAGC | ||
| N1_probe | CAATAACTGGCGCAGCAAACATACCATAC |