TABLE 5.
F. tularensis subspecies and/or subtype singleplex qPCR differentiation assay, chromosomal target, and limit of detection
| Singleplex qPCR assaya | Organism detected | Chromosomal target (locus tag)b | LODc |
|---|---|---|---|
| U16S | Bacteria | 16S rRNA gene | ∼0.1d |
| 4Pan | F. tularensis subsp. tularensis (type A) | ostA1 (FTT_0467) | 3 |
| F. tularensis subsp. holarctica (type B) | |||
| F. tularensis subsp. mediasiatica | |||
| F. tularensis subsp. novicida | |||
| 3Pan | F. tularensis subsp. tularensis (type A) | Hypothetical gene (FTL_1858) | 5 |
| F. tularensis subsp. holarctica (type B) | |||
| F. tularensis subsp. mediasiatica | |||
| A1d | F. tularensis subsp. tularensis subtype A.I | Hypothetical gene (FTT_0516) | 7 |
| A2c | F. tularensis subsp. tularensis subtype A.II | mviN (FTW_1702) | 5 |
| B2 | F. tularensis subsp. holarctica (type B) | Hypothetical gene (FTS_0806) | 5 |
| M3 | F. tularensis subsp. mediasiatica | Hypothetical gene (FTM_1104) | 2 |
| N1 | F. tularensis subsp. novicida | Metabolite H+ symporter (FTN_0003) | 3 |
a
Hydrolysis probes used in the singleplex qPCR assays were labeled with the fluorophore 5′ FAM and 3′ quencher BHQ1.
b
Locus tag prefix with associated F. tularensis strain: FTT, SCHU S4 (subtype A.I); FTW, WY96-3418 (subtype A.II); FTL, LVS (attenuated type B); FTS, FSC200 (type B); FTM, FSC147 (F. tularensis subsp. mediasiatica); FTN, U112 (F. tularensis subsp. novicida).
c
Units are fg except where indicated.
d
Unit is pg.