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. 2020 Feb 13;5(3):e131596. doi: 10.1172/jci.insight.131596

Figure 4. Abrogation of SLC2A5 impairs fructose uptake and fructose-induced cell proliferation of LC cells in vitro.

Figure 4

(A) Western blot showing CRISPR/Cas9-mediated deletion of SLC2A5 in A549 and EKVX cells. (B) Fructose uptake by A549 and EKVX cells with or without SLC2A5 ablation in vitro. Statistical analysis was conducted using 1-way ANOVA test. After carrying out a homogeneity of variance test to confirm equal variance among subgroups, P values were acquired from post hoc test using LSD method. Cumulative data are shown; n = 3. (C) Fructose-induced cell proliferation of A549 and EKVX cells with or without SLC2A5 abrogation in vitro. Cells were cultured in fructose medium for 72 hours. Statistical analysis was conducted using 1-way ANOVA test. After performing a homogeneity of variance test to verify equal variance among subgroups, P values were gained from post hoc test using LSD method. Cumulative data are shown; n = 3. NC, nontarget control; KO, knockout. Data shown as mean ± SEM. ***P < 0.001, 2-tailed Student’s t test.