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. 2020 Feb 13;5(3):e133972. doi: 10.1172/jci.insight.133972

Figure 1. Stiff matrix inhibits expression of the CFIm subunits at the mRNA and/or protein level.

Figure 1

Primary lung fibroblasts were isolated from failed donor lungs of 3 human subjects (subj). Cells were cultured on soft and stiff polyacrylamide gels for 48 hours. (A) Relative levels of CFIm68, CFIm59, and CFIm25 mRNA were determined by real-time RT-PCR. GAPDH was used as internal reference control. Bar graphs represent (mean ± SD) 5 separate experiments. (B) Protein levels of CFIm68, CFIm59, and CFIm25 were determined by immunoblot. GAPDH was used as loading control. Densitometry was performed using ImageJ (NIH). Relative density of CFIm subunits was normalized to GAPDH. Bar graphs represent (mean ± SD) 3 separate experiments. A 2-tailed Student’s t test was used for comparison between groups.