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. 2020 Feb 13;5(3):e133972. doi: 10.1172/jci.insight.133972

Figure 7. Effects of knockdown of CFIm59 on matrix stiffness–regulated APA and expression of COL1A1 and FN1.

Figure 7

(A) CFIm59-specific siRNA (si-59) and the control siRNA (si-con) were transfected into human lung fibroblasts. Knockdown of CFIm59 expression was determined by immunoblot. GAPDH was used as loading control. (B–G) Lung fibroblasts transfected with si-59 and si-con were cultured on soft and stiff hydrogels for 48 hours. Ratios of long-to-total COL1A1 (B) and FN1 (E) transcripts were used to evaluate the proximal poly(A) site usage. Relative levels of COL1A1 (C) and FN1 (F) mRNA were determined by real-time RT-PCR. Protein levels of COL1A1 (D) and FN1 (G) were determined by immunoblot. GAPDH was used as internal reference or loading control. Bar graphs represent (mean ± SD) 3 or 4 separate experiments. Statistical analysis was performed by 1-way ANOVA.