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. 2020 Feb 13;5(3):e131277. doi: 10.1172/jci.insight.131277

Figure 4. Blockade of IL-17A ameliorates alcohol-induced brain injury and neuroinflammation.

Figure 4

Brains and cerebrospinal fluid (CSF) were collected from IG alcohol–fed WT mice with or without RORγt inhibitor or with or without anti–IL-17A Ab (male C57BL/6, 12 weeks old, n = 12/group, 3 independent experiments). (A) Coronal brain sections were immunostained for induction of brown adipocytes 1 (Iba1) (hippocampus, ×20 and ×100 objectives) or glial fibrillary acidic protein (GFAP) (cortex, ×4 and ×40 objectives). (B) Positive area was calculated as percentage. (C) Expression of Iba1 and GFAP mRNA. Data are shown as fold change. (D) CSF levels of IL-17A and TNF-α were measured by ELISA (pg/mL). (E) Expression of TNF-α mRNA was measured. Two-tailed Student’s t test and 1-way ordinary ANOVA for multiple comparisons were applied. *P < 0.05 (see Supplemental Figures 7–10).