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. 2020 Feb 13;5(3):e131277. doi: 10.1172/jci.insight.131277

Figure 6. Inhibition of IL-17A suppresses voluntary alcohol drinking in alcohol-dependent mice.

Figure 6

(A and B) Voluntary alcohol intake (g ethanol/kg body weight) was measured during 2BC sessions (2 hours) in Air and CIE mice with or without anti–IL-17A Ab (or with or without RORγt inhibitor). (A) Multiple doses of anti–IL-17A Ab were administered on consecutive days (the regimen is shown, red arrows). The most significant effect was detected on the first day of treatment (withdrawal day 4: effect of Ab, F[1,19] = 6.6, and P < 0.05; effect of CIE, F[1,19] = 9.7, and P < 0.01; Ab by CIE interaction, F[1,19] = 5.1, and P < 0.05) and persisted throughout the week (withdrawal days 4–7: effect of Ab, F[1,19] = 3.5, and P < 0.08; see Supplemental Figure 10). (B) Air and CIE mice with or without RORγt inhibitor. There was no immediate effect of RORγt inhibitor on voluntary ethanol drinking. The mice were then exposed to an additional week of CIE (or Air) and resumed 2BC sessions. The most significant effect was observed on withdrawal day 3 (effect of inhibitor, F[1,20] = 10.1, and P < 0.01; effect of CIE, F[1,20] = 47.3, and P < 0.001; inhibitor by CIE interaction, F[1,20] = 7.2, and P < 0.05) but persisted throughout the week (withdrawal days 3–6: effect of inhibitor, F[1,20] = 5.5, and P < 0.05). The regimen of IL-17A inhibition is specified (red arrows). One-way ordinary ANOVA for multiple comparison was applied.