Skip to main content
. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Environ Sci Pollut Res Int. 2019 Nov 12;27(9):8897–8904. doi: 10.1007/s11356-019-06663-0

Figure 2:

Figure 2:

Knockdown of the aryl hydrocarbon receptor (AhR) gene protects from the anti-beiging effects of PCB126 or IS. A. Preadipocytes expressing scrambled (SCR) shRNA (shSCR) of shRNA against AhR (shAHR) were treated with vehicle or PCB126 at concentrations of 2 or 10 μM for 4 days after the PCB126 was removed followed by differentiation for 10 days. Differentiated adipocytes were treated with norepinephrine at 10 μM for 6 hours in media without serum after which the cells were collected for RNA and assessed for UCP1 transcript levels as described in the Materials and Methods. Transcript levels were normalized to GAPDH. Shown values are fold-changes over non-stimulated (no NE) controls. B. Preadipocytes expressing shSCR or shAHR were treated with vehicle or IS at concentrations of 10 or 50 μM for 4 days after the IS was removed followed by differentiation for 10 days. Fold changes in NE-induced UCP1 induction were assessed as in A. For both A and B, values represent 3 replicates. Statistical analysis was performed using the GraphPad Prism program and 2-way ANOVA with Sidak’s multiple comparison test ((* p<0.05; **** p<0.0001; ns=nonsignificant).