Figure 4:

Effects of PCB126 or IS on already-differentiated adipocytes. A. Preadipocytes were differentiated for 10 days followed by treatment with vehicle or PCB126 at 2 or 10 μM for 4 days in non-differentiating conditions. Cells were treated with norepinephrine at 10 μM for 6 hours in media without serum after which the cells were collected for RNA and assessed for UCP1 transcript levels as described in the Materials and Methods. Transcript levels were normalized to GAPDH. Shown values are fold-changes over non-stimulated (no NE) controls. B. Preadipocytes were differentiated for 10 days followed by treatment with vehicle or IS at 10 or 50 μM for 4 days in non-differentiating conditions. Fold changes in NE-induced UCP1 induction were assessed as in A. Statistical analysis was performed using the GraphPad Prism program and one-way ANOVA with Dunnett’s multiple comparison test (** p<0.01; *** p<0.001).