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. Author manuscript; available in PMC: 2020 Mar 27.
Published in final edited form as: Methods. 2018 Jun 28;150:11–18. doi: 10.1016/j.ymeth.2018.06.014

Fig. 4. Cpf1 protein purification.

Fig. 4.

A. Schematic of the different steps required for the production and purification of Cpf1, and the possible interruptions and storage. B. Example of Heparin ion exchange chromatography purification, where Cpf1 is eluted with a gradient of salt KCl (in green). C. Example of size-exclusion chromatography purification of Cpf1. D. Validation of Cpf1 purification by SDS-PAGE electrophoresis.