Figure 3.

Treatments with EGF and/or hGly²GLP-2 increase crypt-villus growth in C57Bl/6 mice. Male (n = 4–5) and female (n = 4–5) 6- to 8-week C57Bl/6 mice were treated for 11 days with various combinations of vehicle, EGF, and/or hGly²GLP-2. (A) Jejunal villus height and crypt depth, (B) Proliferative index in the jejunal transit amplifying zone and (C) Bax:Bcl2 ratio in jejunal mucosal scrapes. All data were normalized by sex prior to combination within each treatment group to make n = 8–9. *P < 0.05, **P < 0.01, ***P < 0.001 as indicated; #P < 0.05, ##P < 0.01 and ###P < 0.001 vs hGly²GLP-2 alone.