Figure 4.
Treatments with EGF and/or hGly2GLP-2 do not affect expression of growth factor ligands or their receptors in C57Bl/6 mice. Male (n = 4–5) and female (n = 4–5) 6- to 8-week C57Bl/6 mice were treated for 11 days with various combinations of vehicle, EGF and/or hGly2GLP-2. Jejunal mucosal scrapes were analysed by qRT-PCR for (A) proglucagon (GCG), (B) GLP-2R, (C) IGF-1, (D) IGF-1R, (E) EGF, (F) ErbB1, and (G) ErbB2 transcripts, as normalized to expression of 18S rRNA. All data were normalized by sex prior to combination within each treatment group to make n = 8–9.