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. 2020 Jan 31;39(13):2724–2740. doi: 10.1038/s41388-020-1178-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Western blots showing EPHA2 knockdown in AGS and MKN45 cells. b Cell viability was not significantly affected by EPHA2 knockdown in AGS and MKN45 cells analyzed by MTT assays. Cells were transfected with two independent siRNAs (si-EPHA2-1 and si-EPHA2-2) or nontargeting control siRNA (si-Control). Data are presented as mean (n = 3) ± SD. c Cell migration was analyzed using transwell migration assays. Data are presented as mean (n = 3) ± SD. *p < 0.05; **p < 0.01. d Cell invasion was analyzed using Matrigel invasion assays. Data are presented as mean (n = 3) ± SD. *p < 0.05. e Western blots showing stable EPHA2 knockdown in AGS and MKN45 cells. f Tumor sizes (left panel) and weights (right panel) were not significantly affected by EPHA2 knockdown in NOD/SCID mice subcutaneously injected with AGS cells. Mice were sacrificed at day 45 (n = 5 for each group). This experiment was conducted once. g Tumor sizes (left panel) and weights (right panel) were not significantly affected by EPHA2 knockdown in NOD/SCID mice subcutaneously injected with MKN45 cells. Mice were sacrificed at day 30. (n = 5 for each group) This experiment was conducted once. h EPHA2 knockdown decreased lung metastasis in NOD/SCID mice intravenously injected with AGS cells. Numbers of tumor nodules in lungs are shown as means ± SD. *p < 0.05. This experiment was conducted once. i Representative images of tumor formation in NOD/SCID mice intraperitoneally injected with MKN45 cells. Mice were sacrificed at day 30. Blue arrowheads indicate tumor nodules. j Statistical results of NOD/SCID mice intraperitoneally injected with MKN45 cells. EPHA2 knockdown significantly decreased numbers of tumor nodules (left panel) and total tumor weights (right panel). *p < 0.05. This experiment was conducted once. All statistical data were analyzed and obtained through Student’s t test.