FIGURE 1.

The effect of calcium restitution and citrate-anticoagulants in endothelial proliferation and migration mediated by PRPr. (A,B) Platelets contained in SC-PRP and ACD- were lysed to release total amount of platelet-derived growth factors and then coagulated or not with CaCl₂. Clot was removed and PRPr releasates (PRPr) were incubated with HMEC-1. SC-PPP and ACD- coagulated or not with CaCl₂ were used as control. Endothelial cell proliferation at 24 h (A) and migration at 8 h (B) were determined. Images are representative of seven independent experiments and were obtained before and after treatments (magnification 40X). (C–D) HUVEC were incubated with SC-PRPr and ACD- supplemented or not with CaCl₂. SC-EGM2 or ACD- supplemented or not with CaCl₂ was used as control. Endothelial proliferation at 24 h (C) and migration at 8 h (D) were determined (n = 4–7; *P < 0.05, **P < 0.01, ***P < 0.001 vs. CaCl₂ (-); ^#P < 0.05, ^##P < 0.01 vs. SC-PRPr or SC-EGM2. Two-way ANOVA followed by Fisher test).