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. 2020 Mar 20;11:473. doi: 10.3389/fimmu.2020.00473

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Copyright © 2020 Xie, Yang, Wu, Ma, Wei, Fei and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Trib1 knockdown on cell proliferation and apoptosis of renal tubular epithelial cells in vitro. (A) The knockdown efficiency of lentivirus-mediated transfection of Trib1-shRNA as analyzed by western blotting in renal tubular epithelial cells. (B) Cell viability as measured by CCK8 assay in renal tubular epithelial cells transfected with NC-Trib1 and Trib1-shRNA. (C) Protein expression of BAX, Bcl-2 and Cyclin B as detected by western blotting. (D) Cell apoptosis induced by Trib1 knockdown as determined by flow cytometry. (E) Quantitative analysis of apoptotic cell proportion in panel d. * indicates the NC-Trib1 group vs the Trib1-shRNA group. *p < 0.05; **p < 0.01.