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. 2020 Mar 20;14:38. doi: 10.3389/fncel.2020.00038

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Copyright © 2020 Chen, Tian, Luo, Xiao, Li and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification and purity of SMNs by immunofluorescence with motor neuron-specific markers against SMI-32 and CHAT. The immunofluorescence assay of motor neuron marker SMI-32 (green, A) and CHAT (red, B) showed cell bodies, dendrites, and large axons, with 4′,6-diamidino-2-phenylindole (DAPI) staining (blue, C) showing the nuclei. (D) Merged image. (E) The percentage of SMI-32 or CHAT-immuopositive cells were calculated. Data were presented as mean ± SD (n = 5 independent experiments). At least 10 random fields from one sample were averaged in each independent experiment. Scale bars represent 20 μm.