Skip to main content
. 2020 Mar 20;14:38. doi: 10.3389/fncel.2020.00038

Figure 4.

Figure 4

Morphological changes and cell viability of cultured SMNs under OGD for different time. Primary SMNs were cultured normally (A) or subjected to OGD for various periods of time (B: 1 h, C: 3 h, D: 5 h, E: 7 h, or F: 24 h, respectively), and the representative phase photomicrographs showed the morphologic changes (×250). (A–F) OGD resulted in destruction of neural networks, and the severity increased with the prolonging of OGD time. (G) Cell viability was reduced under OGD in a time-dependent manner. The cell viability was assessed using the CCK8 kit, and results were expressed as the percentage of CCK8 reduction. The absorbance of control cells was set at 100%. Data were presented as mean ± SD (n = 5 independent experiments). Statistical comparisons were carried out with one-way analysis of variance (ANOVA). *p < 0.05 compared with control group. #p < 0.05 between groups.