Figure 6.

Double immunofluorescence stainings show the LC3-positive puncta in primary SMNs under OGD. (A) Representative fluorescent confocal images of cells counterstained with DAPI (blue, nuclei), autophagy-specific marker—LC3 (green) as well as motor neuron marker—CHAT (red) are shown (n = 3 independent experiments). Strong, punctate LC3 staining can be observed in primary SMNs under OGD. Scale bar presents 10 μm. (B) The relative fluorescent intensity of LC3 was analyzed using ImageJ. Data were presented as mean ± SD (n = 3 independent experiments). Statistical comparisons were carried out with unpaired Student’s t-test. *p < 0.05.