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. 2020 Mar 20;14:38. doi: 10.3389/fncel.2020.00038

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Copyright © 2020 Chen, Tian, Luo, Xiao, Li and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LC3 expression under OGD with Baf-A1 to observe the real state of autophagic flux. (A) The expression of LC3 I and LC3 II were detected by Western blot. In the presence of Baf-A1, LC3 II dramatically increased and accumulated. (B) The quantitative results are shown in the bar chart. Data were presented as mean ± SD (n = 3 independent experiments). Statistical comparisons were carried out with one-way ANOVA. *p < 0.05 compared with the control group. ^#p < 0.05 compared with the 7-h group.