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. 2020 Mar 20;11:274. doi: 10.3389/fphar.2020.00274

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Copyright © 2020 Law, Wright, Iqbal, Norling and Cooper.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Macrophages are a source of Gal-1 in the peritoneal cavity. Peritoneal lavages were performed on C57/Bl6 naive mice (0 h) or 4, 24, and 48 h following administration of zymosan (1mg in 500 μl DPBS^+/+ i.p.) and cell free supernatants were assayed for Gal-1 concentration by ELISA (A). Total Gal-1 levels were assessed in permeabilized monocytes/macrophages (identified using Ly6C⁺, F4/80⁺) by flow cytometry (B). Statistical analysis was performed using a one-way ANOVA with Tukey’s multiple comparisons test, results are displayed as mean ± SEM, and in all cases, significant results are considered as *P < 0.05, with ***P < 0.0005 and ****P < 0.0001. n = 3–4 mice per group.