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. 2020 Mar 27;5:23. doi: 10.1038/s41392-020-0105-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b Triglyceride and cholesterol levels measured by the glycerol lipase oxidase (GPO-PAP) method in Huh7 and Hep-G2 cells treated with different concentrations of TAC. c FASN, SREBP2, and AKT protein expression in Huh7 and Hep-G2 cells analyzed by western blotting. d Body weight of mice assessed every week in the control (black), low-dose TAC (red), and high-dose TAC (blue) mouse groups (n = 8 per group). e, f Serum total cholesterol (TC) and triglycerides (TG) assessed 8 weeks after the initial TAC injection. g Hepatic triglyceride levels were measured by Oil red O staining. h FASN, SREBP2, and AKT protein expression in mouse liver was analyzed by western blotting. Data represent the mean ± SD. *P < 0.05, **P < 0.01, NS, not significant, TAC groups vs. control. The experiments were independently repeated at least three times.