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. 2020 Mar 27;5:23. doi: 10.1038/s41392-020-0105-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Transfection efficiency of miR-33a mimic in Huh7 and Hep-G2 cells measured by qPCR. b, c Triglyceride and total cholesterol levels in Huh7 and Hep-G2 cells increased in the miR-33a mimic groups when measured by the glycerol lipase oxidase (GPO-PAP) method. d Triglyceride levels in Hep-G2 cells were measured by Oil red O staining. e Protein levels of a few known miR-33a target genes in Huh7 and Hep-G2 cells analyzed by western blotting. Data represent the mean ± SD. *P < 0.05, **P < 0.01, NS, not significant. The experiments were independently repeated at least three times.