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. 2020 Mar 27;5:23. doi: 10.1038/s41392-020-0105-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a circFASN was successfully overexpressed in Huh7 and Hep-G2 cells. b The linear form of FASN was not affected by the circFASN overexpression plasmid. c Relative expression of miR-33a, detected by qPCR, was reduced in the circFASN overexpression (OE) groups in Huh7 and Hep-G2 cells. d circFASN was silenced in Huh7 and Hep-G2 cells. e The linear form of FASN was not affected by silencing circFASN. f The relative expression of miR-33a, detected by qPCR, was increased in the circFASN-silenced Huh7 and Hep-G2 cells. g, h Triglyceride levels in Huh7 and Hep-G2 cells measured by the glycerol lipase oxidase (GPO-PAP) method. i Protein levels of ABCG1 and CPT1A in Huh7 and Hep-G2 cells determined by western blotting. Data represent the mean ± SD. *P < 0.05, **P < 0.01, NS, not significant. The experiments were independently repeated at least three times.