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. 2020 Mar 27;5:23. doi: 10.1038/s41392-020-0105-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Triglyceride levels in Hep-G2 cells measured by Oil red O staining in the negative control (NC), TAC only -treated, and TAC and circFASN OE plasmid-treated groups. The ratio of the area of lipid droplets to that of a single cell was used to evaluate lipid content in cells. b, c Relative expression of circFASN and miR-33a in the NC, TAC, and TAC+OE groups detected by qPCR. d Protein expression of FASN and SREBP2 in Huh7 and Hep-G2 cells analyzed by western blotting. Data represent the mean ± SD. *P < 0.05, **P < 0.01, NS, not significant, TAC groups vs. the control or OE group. The experiments were independently repeated at least three times.