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. 2020 Mar 26;10:5544. doi: 10.1038/s41598-020-62506-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BFT increases tight-junction related protein in the colon and prevents from metabolic endotoxemia in HF-fed mice. (A) Relative short-chain fatty acid levels in the cecum of HFD (white) or HFD + BFT (black). (B) Fecal mucin content in mice treated either chow, HFD or HFD + BFT. (C) FITC levels in serum of mice after gavage administration of FITC-dextran. The Figure in the upper square is relative Akkermansia DNA levels of mice on a HFD or HFD + BFT used for the experiments (n = 7–8). (D) Western blots for claudin1 in the colon of mice on a chow or an HFD with or without BFT (18 weeks of age, 12 weeks on the HFD, 8 weeks on BFT) and quantitation of claudin1 protein normalized to β-actin (n = 3 per group). (E) Lipopolysaccharide (LPS) levels in serum of mice fed on an HFD with either saline or BFT for 12 weeks (n = 6–7). (F) Lbp mRNA levels in the liver (n = 7). (G) Lyz1 and F4/80 mRNA levels in the colon (n = 8–13). *p < 0.05 and **p < 0.01, by unpaired, 2-tailed t test for (A,C,E,F,G) or ANOVA, followed by Turkey-Kramer post-hoc for (B,D).