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. 2020 Mar 26;10:5544. doi: 10.1038/s41598-020-62506-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BFT decreases adipose tissue inflammation and improved steatohepatitis in HFD-fed mice. qPCR of macrophage and inflammation related genes in epididymal adipose tissues (A) and inguinal adipose tissue (B) of mice treated with either saline (white) or BFT (black) on an HFD for 8 weeks (n = 11–22). (C) Immunohistochemistry of epididymal adipose tissue for F4/80 and collagen I. (D) Quantification of crown like structures (CLS). (E) H&E staining and Sirius Red staining of the liver of chow, HFD or HFD + BFT treated mice. The arrows indicate Sirius Red positive area. (F) Liver weight of chow, HFD or HFD + BFT fed mice. *p < 0.05 and **p < 0.01, by unpaired, 2-tailed t test for (A,B), or ANOVA, followed by Turkey-Kramer post-hoc for (D,F).