Fig. 7. Homeostatic expression of host CCL5 is important for B16-F10 metastasis.
a B16-F10 melanoma cells were intravenously injected into Ccl5+/+, Ccl5ΔPE/ΔPE, and Ccl5ΔDE/ΔDE mice. Representative macroscopic images of lungs 14 days after injection are shown. The summary graph on the right shows the numbers of metastatic nodules. Error bars indicate Mean ± SD and each dot represents a mouse examined over at least two independent experiments. Statistical significance is measured via unpaired two-tailed Student’s t tests and is presented as follows: ***p < 0.001, ****p < 0.0001, ns nonsignificant. b Sequences of the Runx3ΔY allele confirmed the removal of the last tyrosine residue from the RUNX3 protein in Runx3ΔY/ΔY mice. The TAC codon for tyrosine was changed to a TAA stop codon (upper). Contour plots showing CCL5 expression in T and NK cells of Runx3+/+ and Runx3ΔY/ΔY mice (bottom). One representative plot of four independent mice are shown. Numbers in the plots indicate the percentage of cells in each quadrant, and numbers in parentheses indicate the mean fluorescent intensity of CCL5. c The result of B16-F10 melanoma lung metastasis in Runx3+/+ and Runx3ΔY/ΔY mice is shown as in a. Error bars indicate Mean ± SD and each dot represents a mouse examined over at least two independent experiments. Statistical significance is measured via unpaired two-tailed Student’s t tests and is presented as follows: *p < 0.05, ***p < 0.001. d The result of YAC-1 cancer model. PKH67-labeled mouse lymphoma YAC-1 was injected i.v. into Ccl5+/+ and Ccl5ΔPE/ΔPE mice and survived cancer cells in lungs were counted. Error bars indicate Mean ± SD and each dot represents a mouse examined over at least two independent experiments. Statistical significance is measured via unpaired two-tailed Student’s t tests and is presented as follows: ∗∗∗p < 0.001. Source data are provided as a Source Data file.