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. 2020 Mar 26;10:5515. doi: 10.1038/s41598-020-62240-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The spatiotemporal relationships among mitochondria, autophagosomes, and acidic vesicles. (a) Representative enlarged time-lapse images of granular cells labelled with Hoechst 33258 (blue), CYTO-ID (green), and TMRE (red). The upper, middle, and lower panels show merged images, CYTO-ID signals, and TMRE signals, respectively. The data are representative of 17 cells (4 experiments). (b) Representative enlarged time-lapse images of granular cells labelled with Hoechst 33258 (blue), CYTO-ID (green), and MTR (red). The upper, middle, and lower panels show merged images, CYTO-ID signals, and MTR signals, respectively. The data are representative of 32 cells (4 experiments). (c) Representative time-lapse images of granular cells labelled with CTB (blue), MBG (green), and LTR (red). A dashed line indicates the region for analysis of Pearson’s correlation coefficient in e. (d) An image of MBG signals in c at 52.5 min before the disappearance of mitochondrial signals. Small red rectangles show the mitochondrial regions analysed in e. (e) A graph of the intensity of MBG signals in each region of the rectangles (#1–9) in d and Pearson’s correlation coefficient for the colocalization of LTR signals versus time (red dashed line). The data presented in c–e are representative of 19 cells (5 experiments). Scale bar, 2.5 μm (a,b) or 10 μm (c,d).