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Target-specific long length DNA insertion via CRISPR-Cas9-mediated knock-in method. (A) Schematic diagram of large DNA fragment insertion in CrFTSY. Target-specific DNA cleavage via CRISPR-Cas9 and insertion of the large gene of interest (3.2 kb, GLuc-aph7 DNA cassette) simultaneously. (B) Sequence analysis done by the Sanger sequencing method to identify the inserted DNA fragment on ΔCrFTSY-Ga.
