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. 2020 Mar 20;11:306. doi: 10.3389/fpls.2020.00306

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Copyright © 2020 Kim, Lee, Baek and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Target specific 6.4 kb long DNA (aph8–mVenus–aph7 DNA cassette) insertion via CRISPR-Cas9-mediated knock-in. (A) Sequence analysis of target site and inserted DNA fragment. (B) Visualization of mVenus expression in ΔCrFTSY_mV at insertion site by fluorescence microscopy (red, chlorophyll; green, mVenus). Scale bar indicates 10 μm.