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Experimental design – Coffea arabica cv. Mundo Novo leaves were either treated with the PRIs, ASM (A) and Greenforce CuCa (G) or subsequently inoculated with Hemileia vastatrix (Ai, Gi). Leaves were collected at 3, 5, and 7 days after PRIs treatment (black timeline) and at 2 and 4 days after H. vastatrix inoculation (orange timeline). Leaves treated with water were used as control: mock-treated/inoculated (C) or inoculated (Ci).
