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. 2020 Mar 26;10:5523. doi: 10.1038/s41598-020-62497-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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mRNA and protein expression levels of MUC2 in SPFC-treated LS 174T cells. The cells were treated with RCM or SPFC (10%, v/v) and incubated for 48 h. (A) mRNA expression level determined by qPCR; (B) MUC2 concentration in the supernatant of the cell culture determined by ELISA; (C) images of immunofluorescence-stained cells; (D) quantification results of MUC2 production by immunocytochemistry. mRNA expression levels were normalized to the reference gene GAPDH. Each value indicates the mean ± SD of three independent experiments performed in triplicate. The significance of the differences was determined by the Student’s t-test. NC: the negative control group; RCM: the reinforced clostridial medium-treated group; SPFC: the supernatant of P. freudenreichii culture-treated group.