Figure 4.

(a–c) Representative ¹H spectra and voxel position in the striatum of sham mice and MCAO mice 1 h and 2 h post-reperfusion. (d–f) Time course of selected metabolites after 30 min MCAO surgery (red) and sham operated mice (black); time zero indicates the beginning of reperfusion. Open symbols are individual animals, with Cramer-Rao lower bounds (CRLB) of the LC-model quantification as error bars. Full red circles are the average of all three MCAO animals and full black squares are the average of all three shams -with the grey area representing the corresponding standard deviation. Horizontal lines that designate the metabolite concentration in healthy 8-week C57BL6/J mice as reported by Tkac et al.⁴⁹ were added to guide the eye on the dynamic evolution of the metabolite concentrations. Vertical lines indicate the times at which HP lactate was injected in the HP ¹³C MRS measurements.