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. 2020 Mar 26;10:5507. doi: 10.1038/s41598-020-62319-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in MCT expression patterns after ischaemia-reperfusion. The images in red in the upper panels show the expression of lactate transporters MCT1 (a), MCT2 (b) and MCT4 (c) in the striatum of sham animals and in the striatum ipsilateral and contralateral to the lesion of MCAO mice at 1 h or 2 h post-reperfusion. The lower panels show the merged images of the triple immunolabelling of the MCTs (red) with the neuronal marker MAP-2 (green) to identify the lesion area (i.e. loss of MAP-2 staining), the endothelial cell marker CD31 to identify blood vessels (grey) and the nuclear counterstaining (DAPI, blue). Arrowheads indicate blood vessels. Arrows in (c) indicate patches of high MCT4 expression. Scalebar: 100 μm.