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. 2020 Mar 26;10:5489. doi: 10.1038/s41598-020-62453-6

Table 1.

Univariate associations of potential risk factors with seropositivity to Rift Valley fever virus in cattle, sheep and goats in central South Africa, 2015–2016.

Variable and level Number of animals sampled Number testing positive % seropositive P-value
District* <0.001
  (19 districts) 3,049 622 20.4
Year sampled* 0.019
  2015 2773 550 19.8
  2016 276 72 26.1
Species* <0.001
  Cattle 977 311 31.8
  Sheep 1549 255 16.5
  Goats 523 56 10.7
Breed* <0.001
  Indigenous 1132 153 13.5
  Exotic 1043 258 24.7
  Cross 585 163 27.9
Age* <0.001
  <2y 929 40 4.3
  2–4y 811 97 12.0
  >4y 1294 484 37.4
Sex* <0.001
  Female 2704 590 21.8
  Male 343 32 9.3
Animal born on farm* 0.142
  No 464 104 22.4
  Yes 2262 438 19.4
Animal vaccinated against RVF* <0.001
  No 1892 278 14.7
  Yes 626 226 36.1
  Unknown 531 118 22.2
Type of farm* 0.001
  Communal 308 17 5.5
  Private 2729 604 22.1
Production system* <0.001
  Commercial 1790 436 24.4
  Semi-commercial 733 115 15.7
  Feedlot 202 53 26.2
  Communal 308 17 5.5
Main industry* 0.001
  Meat 1711 361 21.1
  Wool 663 147 22.2
  Dairy 62 28 45.2
  Other 289 68 23.5
Animals mix with other domestic ruminants 0.279
  No 1647 349 21.2
  Yes 1389 274 19.6
Animals mix with wildlife* 0.001
  No 1592 290 18.2
  Yes 1444 331 22.9
Animals kraaled at night* <0.001
  No 2232 511 22.9
  Yes 751 97 12.9
Contact with animals on another farm 0.699
  No 2402 495 20.6
  Yes 635 126 19.8
Animals have access to perennial spring, lake or pond 0.263
  No 1836 363 19.8
  Yes 1150 247 21.5
Animals have access to perennial river or stream* 0.011
  No 1992 380 19.1
  Yes 994 230 23.1
Animals have access to seasonal pan* <0.001
  No 1923 338 17.6
  Yes 1063 272 25.6
Animals have access to manmade water source (dam) 0.405
  No 761 147 19.3
  Yes 2225 463 20.8
New cattle brought onto farm in past 12 months* <0.001
  No 1956 362 18.5
  Yes 1074 259 24.1
New goats brought onto farm in past 12 months* 0.040
  No 2688 565 21.0
  Yes 345 56 16.2
New sheep brought onto farm in past 12 months 0.272
  No 2026 403 19.9
  Yes 1007 218 21.6
Quarantine practiced when introducing animals* 0.014
  No 2276 442 19.4
  Yes 757 179 23.6
Vehicles cleaned and disinfected before and after transporting animals* <0.001
  No 1637 290 17.7
  Yes 1396 331 23.7
Mosquito or fly control practised* 0.103
  No 1353 259 19.1
  Yes 1680 362 21.5
Animals slaughtered on farm* 0.071
  No 825 201 24.4
  Yes 1900 403 21.2
Year of last RVF vaccination on farm* 0.003
   Never 2018 387 19.2
  2009–2011 442 90 20.4
  2012–2013 263 51 19.4
  2014–2016 308 90 29.2
  Unknown 18 4 22.2
Farm size 0.331
  <400 ha 565 108 19.1
  400–1000 ha 665 133 20.0
  1001–3000 ha 1021 232 22.7
  >3000 ha 714 148 20.7
Total number of animals on farm* 0.001
  1–100 689 103 14.9
  101–300 690 145 21.0
  301–1000 741 165 22.3
  >1000 929 209 22.5
Any animals aborted in past 3 months** 0.001
  No 2290 438 19.1
  Yes 743 183 24.6
RVF ever confirmed on farm** 0.027
  No 1844 386 20.9
  Yes 881 218 24.7

P-value for Fisher’s exact test.

*Variable selected for inclusion into multiple logistic regression model (P < 0.20).

**Variable considered a consequence rather than a potential risk factor, therefore not considered for multivariable model.