Table 3.

Effect of Tartrazine mixed with rice, milk or butter on trypsin and chymotrypsin activities using the in vitro digestion

	Tartrazine dose (mg)	Rice	Milk	Butter
Trypsin activity (U/ml of digesta)	0	0.23 ± 0.00	0.19 ± 0.00	0.23 ± 0.01
	7.5	0.23 ± 0.00	0.20 ± 0.00	0.22 ± 0.01
	75	0.24 ± 0.01	0.20 ± 0.01	0.23 ± 0.02
Chymotrypsin activity (U/ml of digesta)	0	0.24 ± 0.00	0.31 ± 0.02	0.37 ± 0.01
	7.5	0.22 ± 0.00	0.31 ± 0.01	0.37 ± 0.01
	75	0.21 ± 0.02	0.29 ± 0.01	0.37 ± 0.01

Data expressed as mean ± SEM (n = 6)

*Significant at p < 0.05 compared to control using one-way analysis of variance (ANOVA) followed by Tukey multiple comparisons test